The Influence of Media Composition on Root Induction of Patchouli (Pogostemon Cablin Benth)

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Eliza Mayura
Ryan Budi Setiawan
Gustian Gustian
Reni Mayerni

Abstract

Patchouli has good prospects for development and plays a role as one of the country's foreign exchange-producing commodities and a source of income for farmers. One of the efforts to improve the genetic quality of patchouli by collecting patchouli germplasm is local, both in production centers and other areas. An alternative method of propagation of superior seeds in a relatively short time can be done through tissue culture. This study aims to determine the composition of the effective media for root formation in patchouli plants. The research was carried out from January to April 2020 at the Tissue Culture Laboratory, Faculty of Agriculture, Andalas University, Padang. The study used a randomized design with 2 treatment factors Media and Accession consisting of 12 treatments with 3 replications. The results showed that there was an interaction between media composition and patchouli accession where the administration of 1 ppm IBA to Rimbo Binuang accession showed the percentage of root formation (100%), the number of roots (11.0 strands), and root length (8.33 cm) but there was no interaction. on the variable when roots appeared but on a single factor, the composition of the media showed a significant difference when roots appeared on the administration of activated charcoal (26.33 days), the administration of 3 ppm IBA (24 days), the administration of 1 ppm of IBA (22 days) and the administration of NAA. 1.5 ppm (24.22 days ).

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